Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

A single cardiomyocyte is a vital tool in the cellular and subcellular level studies of cardiac biology diseases as fundamental unit contraction electrical activity. Hence, isolating viable, high-quality cardiomyocytes from heart initial most crucial experimental step. Comparing various protocols for adult mice, Langendorff retrograde perfusion successful reproducible method reported literature, especially ventricular myocytes. However, quality atrial myocytes perfused remains challenging, few isolation reports are available. Solving this complicated problem extremely important because apart disease, disease accounts large part diseases. Therefore, further investigations on to reveal mechanisms warranted. In paper, protocol based introduced some modifications depth aorta cannulation steps that may affect digestion process isolate were simultaneously made. Moreover, isolated confirmed be amenable patch clamp investigation.